Long Read de novo Assembly with Flye (external plugin)įast de novo assembly of your raw Oxford Nanopore and PacBio long read sequences using Flye, suited to a wide range of applications including both single genome projects and metagenome assembly. Coding skills are not required, though a good understanding of command line interfaces and file formats is.
More accurate annotation of CDS features onto your plasmidsĪutomatic annotation of plasmids is now smarter with adjustment of CDS boundaries to match the nearest valid ORF when annotating single-interval CDSs from your reference features database.Īdd your favorite tools to Geneious with improved Wrapper Plugin systemĬan’t wait for us to add your favorite command line aligner, tree builder or assembler? You might be able to add it yourself from right inside Geneious using the Wrapper Plugin Creator plugin. Optionally eliminate rare codons and restriction sites. New codon optimization algorithm lets you match the codon usage from your organism of choice by generating a new sequence, starting from either a protein or nucleotide sequence. The HMM alignment engine improves both quality and speed of alignments compared to the older ClustalW aligner.īetter back translate and codon optimization Scale up your alignments with Clustal Omega, now included with Geneious Prime. Longread sequence mapping with Minimap2 (external plugin)įast and easy alignment of Oxford Nanopore and PacBio data to a reference sequence using an industry recommended tool, without the hassle of the command lineĬlustal Omega replaces ClustalW for better, faster alignments Quickly add or edit annotations in batch by copying properties from other annotations, sequences or documents. Improve your cloning workflows by quickly determining CDS annotations with premature stop codons. Live numbering can now be shown alongside the translated amino acids on nucleotide sequences.Īdjust either end of the selected region in the Sequence View by dragging.Įasily view multiple sequences with display of restriction enzymes in lane headings, and quick access to an exportable list of fragments.Įasily view and compare CRISPR cut sites between your guide RNAs.
Locate documents quickly in any table with the new filter button added to the status bar. Quickly visualize restriction sites affected by Dam, Dcm and EcoKI methylation. Highlighted methylation sites for restriction enzymes 2016 CRISPR on-target scoring algorithm.Įasily sort and export your protein and nucleotide statistics within the document table. Enjoy the new look and feel of Geneious Prime with an updated design, including new icons and font.Ĭonfidently design your guide RNAs using the Doench et al.